SRA

Poly(ADP-ribose) polymerase (PARP)-1 is the prototypical PARP enzyme well known for its role in DNA repair and as a pro-inflammatory protein. Since PARP1 is an important co-factor of several other pro-inflammatory proteins, in this study we investigated the possible changes in microbial flora of PARP1 knockout mice. Samples from the duodenum, coecum and feces from wild type and PARP1 knockout C57BL/6J male mice were collected and 16S rRNA genes were sequenced. Based on the sequencing results we reconstructed the microbiome and compared samples throughout the lower part of the gastrointestinal system. Our results show that the lack of PARP1 enzyme did only disturb the microbial flora of the duodenum, where the biodiversity increased in the knockout animals on the species level but decreased on the order level. The most prominent change was the overwhelming abundance of the family Prophyromonadaceae in the duodenum of PARP1-/- animals which disappeared in the coecum and feces where families were spread out more evenly than in wild type animals.